At Which Time Period(s) During PCR Thermocycling Is/are Hottest in Temperature

question 31

Multiple Choice

At which time period(s) during PCR thermocycling is/are hottest in temperature?

• A) during DNA denaturation
• B) during primer annealing
• C) during primer extension/elongation
• D) Both the first and last cycles are hotter in temperature than all other cycles.

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Definitions:

Electron Transport System

A series of complexes that transfer electrons through a membrane to create a gradient used for ATP production.

ATP

Adenosine triphosphate, a crucial molecule in cellular energy transfer that drives various biological processes by providing energy.

Oxygen

A chemical element (O) essential for aerobic respiration in living organisms and accounting for about 21% of Earth's atmosphere.

NADH Reductase

An enzyme involved in the electron transport chain that helps in the transfer of electrons from NADH to other molecules.