Which Procedure Is Not Required When the Shotgun Approach to Sequencing

question 13

Multiple Choice

Which procedure is not required when the shotgun approach to sequencing is modified as sequencing by synthesis, in which many small fragments are sequenced simultaneously?

• A) use of restriction enzymes
• B) sequencing each fragment
• C) cloning each fragment into a plasmid
• D) ordering the sequences
• E) PCR amplification

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Definitions:

Fortified Milk

Milk that has been supplemented with additional vitamins and minerals, such as vitamin D and calcium, for enhanced nutritional value.

Muscle Contraction

Describes the process where muscle fibers actively shorten or generate tension in response to signals from the nervous system.

Potassium

A chemical element (K) essential for the normal functioning of cells, nerves, and muscles.

Pernicious Anemia

A type of anemia caused by the body's inability to absorb vitamin B12, leading to reduced red blood cell production.