Which Procedure Is Not Required When the Shotgun Approach to Sequencing

question 13

Multiple Choice

Which procedure is not required when the shotgun approach to sequencing is modified as sequencing by synthesis, in which many small fragments are sequenced simultaneously?

• A) use of restriction enzymes
• B) sequencing each fragment
• C) cloning each fragment into a plasmid
• D) ordering the sequences
• E) PCR amplification

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Definitions:

Organizing

The act or process of arranging, structuring, or coordinating elements in a systematic way to achieve a specific goal or order.

Formulating Plans

The process of creating strategies or outlining steps to achieve a specific goal, often involving problem-solving and decision-making skills.

Thalamus

A small structure within the brain that relays motor and sensory signals to the cerebral cortex.

Sensory Relay Station

A term typically referring to the thalamus, a structure in the brain that serves as a hub for transmitting sensory information to the cerebral cortex.