The Applications of the Yeast Two-Hybrid Method Include All of the Following

question 64

Multiple Choice

The applications of the yeast two-hybrid method include all of the following EXCEPT

• A) mapping protein-protein interactions in E. coli.
• B) identifying of physiologically significant protein-protein interactions as potential antibiotic targets.
• C) finding unknown proteins ("prey") that interact with proteins of known functions ("bait").
• D) mapping protein-protein interactions in M. tuberculosis.
• E) the ability to probe interactions in vivo.

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Definitions:

Aldol Condensation

A chemical reaction that combines two aldehyde or ketone molecules to form a larger β-hydroxy aldehyde or β-hydroxy ketone, followed by dehydration to give a conjugated enone.

Base-Mediated Aldol Condensation

A reaction process that involves the use of a base to facilitate condensation between two carbonyl compounds, forming a β-hydroxy carbonyl compound or an α,β-unsaturated carbonyl compound.

Enolate

An anion formed by the deprotonation of an alpha-hydrogen of a carbonyl compound, important in many organic reactions.

Haloform Reaction

A chemical reaction where a haloform is produced by the halogenation of methyl ketones in the presence of base.