Enumerating the Viable and Total Cell Concentration of a Population

question 86

Essay

Enumerating the viable and total cell concentration of a population of a microbial isolate can be a laborious task for a microbiologist, especially when studying the same isolate for several years. It is often more practical to determine the relationship between optical density (OD) and cell concentration. Once this relationship (determined by a standard curve) is determined, the OD of an isolate in a broth can be used to determine the populationʹs concentration. Why must a standard curve be prepared for each isolate when using OD measurements to determine cell concentration? Also describe an experiment that would generate this type of standard curve.

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Definitions:

Testosterone

Testosterone is a steroid hormone primarily produced in the testes of males and the ovaries of females, playing a key role in the development of male reproductive tissues and secondary sexual characteristics.

Integumentary System

The organ system comprised of the skin and its appendages, serving to protect the body from various kinds of damage, such as loss of water or abrasion from outside.

Infections

The invasion and multiplication of microorganisms such as bacteria, viruses, and fungi in the body, which can cause illness and disease.

Hormones

Chemical messengers produced by glands in the endocrine system that regulate processes such as growth, metabolism, and reproductive functions.