Identify the Correct Sequence in Which the Steps Below Occur

question 50

Multiple Choice

Identify the correct sequence in which the steps below occur during a single PCR cycle.
1) Complementary base pairing between primers and target DNA.
2) Addition of DNA nucleotides by Taq DNA polymerase.
3) Heat separation of strands of target DNA.

• A) 3; 1; 2
• B) 1; 2; 3
• C) 1; 3; 2
• D) 2; 3; 1
• E) 2; 1; 3

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