If PCR Is Carried Out on a Sample Using Primers

question 51

Multiple Choice

If PCR is carried out on a sample using primers specific for HPV 16 and no DNA is amplified, can a person assume that they are HPV-free?

• A) No. The person likely does not have HPV16 but they may have another HPV strain.
• B) Yes. If there is no HPV16 present, there definitely won't be any other strain of HPV present either.
• C) Yes. Primers specific for HPV 16 would also detect all other strains of HPV, so a negative test indicates the person is free of any HPV.
• D) No. The negative result simply indicates that HPV is not a DNA virus but is in fact an RNA virus.
• E) No. The negative result simply indicates that HPV is not a DNA virus but is in fact a bacterium which has a different type of DNA.

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