Scenario You Conducted a Series of Experiments to Test Whether the the Expression

question 23

Multiple Choice

Scenario
You conducted a series of experiments to test whether the expression of hexokinase, an important enzyme involved in glycolysis, varies under different conditions. In the first experiment, you measured the production of hexokinase mRNA in cells at three different temperatures: 37°C, 25°C, and 10°C. Your results showed that the mRNA levels for hexokinase were 5× and 20× lower at 25°C and 10°C than at 37°C, respectively. Based on these results, you are going to conduct further experiments to attempt to answer your research question.
-DNA microarrays can be used to determine relative levels of gene expression between samples. DNA microarrays are usually interpreted by color: green means higher mRNA levels, yellow means equal levels, and red means lower levels. If you ran the samples from the temperature experiment on a DNA microarray using the 25°C experiment as a baseline, what results do you predict you would obtain?

• A)The samples from the 37°C experiment would be green and the samples from the 10°C would be red.
• B)The samples from the 37°C experiment would be red and the samples from the 10°C would be green.
• C)The samples from the 37°C experiment would be green and the samples from the 10°C would be yellow.
• D)The samples from the 37°C experiment would be yellow and the samples from the 10°C would be red.

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Definitions:

Beta-Amyloid

A protein fragment snipped from an amyloid precursor protein that, in excessive amounts, can form plaques in the brain and is a hallmark of Alzheimer's disease.

Protein Tau

A protein associated with the stabilization of microtubules in nerve cells, whose abnormal accumulation is linked to neurodegenerative diseases like Alzheimer's.

Plaques

Deposits of fatty substances, cholesterol, cellular waste products, calcium, and fibrin in the inner lining of an artery.

Cognitive Reserves

The mind's resilience to withstand neurological damage due to aging or other factors, often linked to an individual's level of mental engagement and education.