You Have Purified a Multisubunit Extracellular Protein That Has Several

question 6

Multiple Choice

You have purified a multisubunit extracellular protein that has several interchain disulfide bonds. Which of the following chemicals would you add to your purified protein mixture if you wanted to eliminate the disulfide bonds?

• A) NaCl, a salt
• B) SDS, an ionic detergent and denaturing agent
• C) H₂O₂, an oxidizing reagent
• D) Tris, a buffering agent
• E) DTT, a reducing agent

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