To Study the Chromatin Remodeling Complex SWR1, a Researcher Has

question 1

Multiple Choice

To study the chromatin remodeling complex SWR1, a researcher has prepared arrays of nucleosomes on long DNA strands that have been immobilized on magnetic beads. These nucleosomes are then incubated with an excess of the H2AZ-H2B dimer (which contains the histone variant H2AZ) in the presence or absence of SWR1 with or without ATP. She then separates the bead-bound nucleosomes (bound fraction) from the rest of the mix (unbound fraction) using a magnet, elutes the bound fraction from the beads, and performs SDS-PAGE on the samples. This is followed by a Western blot using an antibody specific to the H2AZ protein used in this experiment. The results are shown below, with the presence (+) or absence (-) of ATP, SWR1, or the H2AZ-H2B dimer in each incubation reaction indicated at the top of the corresponding lane.
Which of the following statements is confirmed by the Western blot shown?

• A) SWR1 deposits H2AZ histones into the nucleosome arrays.
• B) SWR1 function is not ATP-dependent.
• C) The antibody used in this experiment binds to the SWR1 complex.
• D) All of the above.

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