As Shown in the Following Drawing, a Researcher Has Engineered

question 9

Short Answer

As shown in the following drawing, a researcher has engineered three pairs of LoxP sites (for conservative site-specific recombination) in a region that contains three reporter genes coding for red, yellow, or cyan fluorescent proteins, respectively. Each type of LoxP sequence (shown as a black, gray, or white arrowhead) is specific, meaning it does not recombine with the other types of LoxP sequences. Upon Cre recombinase activation, depending on which recombination event occurs first (which we assume is random), a number of possible combinations of reporters can remain in the final DNA. For each of the following combinations, indicate whether it can (C) or cannot (N) result from this recombination scheme. Do not consider the re-integration of excised DNA, which happens very rarely. Your answer would be a six-letter string composed of letters C and N only, e.g. CCCCNN.

( ) Red and yellow
( ) Red only
( ) Yellow only
( ) Cyan only
( ) Yellow and cyan
( ) Red and cyan

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Definitions:

Photography Series

A collection of photographs focused on a specific subject, theme, or concept, presented as a cohesive body of work.

Corporal Punishment

A form of physical discipline that involves the deliberate infliction of pain as retribution for an offense.

Critical Thinking

The objective analysis and evaluation of an issue in order to form a judgment, involving skepticism, logic, and reasoning.

Motivation Strategies

Techniques or methods used to inspire individuals to initiate or persist in tasks towards achieving goals.