You Have Transfected HeLa Cells with a Gene Encoding the Human

question 18

Short Answer

You have transfected HeLa cells with a gene encoding the human immunodeficiency virus (HIV) protein Rev and have induced the expression of the protein. You incubate the cell culture in the presence or absence of leptomycin B and later measure the localization of Rev inside the cells by immunofluorescence microscopy. Leptomycin B specifically inhibits the cellular Crm1 protein, a nuclear transport receptor that is essential for the normal function of Rev in the HIV life cycle. Your results are tabulated below. Which condition (1 or 2) do you think corresponds to the presence of leptomycin B?

---

Definitions:

Total Revenue

The total amount of money generated by a business from the sale of goods or services before any expenses are subtracted.

Marginal Cost

The change in total cost that arises when the quantity produced is incremented by one unit; essentially, it is the cost of producing one more unit of a good.

Output

The quantity of goods or services produced by a firm or economy.

Total Revenue Curve

A graphical representation that shows how total revenue changes as the quantity sold of a good or service changes, holding all other factors constant.