You Have Transfected HeLa Cells with a Gene Encoding the Human

question 18

Short Answer

You have transfected HeLa cells with a gene encoding the human immunodeficiency virus (HIV) protein Rev and have induced the expression of the protein. You incubate the cell culture in the presence or absence of leptomycin B and later measure the localization of Rev inside the cells by immunofluorescence microscopy. Leptomycin B specifically inhibits the cellular Crm1 protein, a nuclear transport receptor that is essential for the normal function of Rev in the HIV life cycle. Your results are tabulated below. Which condition (1 or 2) do you think corresponds to the presence of leptomycin B?

---

Definitions:

Federal Unemployment Tax Rate

A tax rate set by the federal government to fund unemployment compensation payments to workers who have lost their jobs.

Salaries Payable

Salaries payable is an account that records the amounts owed to employees for work performed but not yet paid.

Employer's Payroll Tax Expense

Taxes that employers are required to pay on behalf of their employees, such as Social Security and Medicare taxes, often based on a percentage of the employees' wages.

Gross Payroll

The total amount of money paid by a business to its employees before any deductions are made.