You Have Used a Variation of Fluorescence in Situ Hybridization

question 61

Short Answer

You have used a variation of fluorescence in situ hybridization (FISH) called flow-FISH. In this technique, DNA probes are labeled with fluorescence and hybridized to their targets inside the cells in a tissue. The cells are then sorted using a fluorescence-activated cell sorter based on their fluorescent signal intensity. The probes in your experiment are designed to hybridize to human telomeric sequences at the ends of the chromosomes. You perform flow-FISH using these probes on two human cell types, an early primary fibroblast culture and a late secondary culture that is showing the signs of replicative cell senescence. The sorting results are presented in the following histograms. Which histogram (1 or 2) would you expect to correspond to the primary culture?

Learn how to modify the appearance and layout of tables in a document.

Grasp the concept of working with paragraph indents and spacings to format document contents effectively.

Acquire the skills to use document formatting tools to align and position tables and text.

Understand how to insert and manipulate graphical elements in a document.

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Definitions:

Quantity Control

Regulatory measures or policies aimed at limiting the amount of goods produced, supplied, or available in a market.

Lower Limit

The smallest value that a given set, statistical sample, or data category can take on or be assigned.

Upper Limit

The maximum level or value that can be reached or is allowable in a given context.

Supply and Demand

Fundamental economic model describing how prices vary as a result of a balance between product availability and consumer demand.