You Would Like to Clone a Gene by PCR

question 1

Multiple Choice

You would like to clone a gene by PCR. The sequence of the two ends of the coding strand for the gene is provided below. Which following pair of primers would direct the amplification of this gene? All sequences are written from 5? to 3?.

ATGAAATCTACGTTTCAC……CCCCCAGTACCCCCCTTA

• A) ATGAAATCTACGTTTCAC ; CCCCCAGTACCCCCCTTA
• B) ATGAAATCTACGTTTCAC ; TAAGGGGGGTACTGGGGG
• C) TACTTTAGATGCAAAGTG; ATTCCCCCCATGACCCCC
• D) TACTTTAGATGCAAAGTG ; CCCCCAGTACCCCCCTTA
• E) GTGAAACGTAGATTTCAT; ATTCCCCCCATGACCCCC

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Definitions:

Newborn

A term referring to an infant in the very first hours or days of their life outside the uterus.

Secondary Sexual Features

Characteristics that distinguish the two sexes of a species, but are not directly involved in reproduction, such as breast development in females and facial hair in males.

Pubescent

Refers to a stage of adolescence in which an individual begins to develop secondary sexual characteristics.

Differentiated Cell

A cell that has developed to perform a specific function, having unique structural attributes distinguishing it from other cell types.