The Binding of Protein a to Two Other Proteins, B

question 66

Multiple Choice

The binding of protein A to two other proteins, B and C, has been studied with fluorescence anisotropy, and the results of the measurements have been plotted in the following schematic diagram. Given equal kₒn rate constants, which complex do you think has a longer half-life? What is the value of K?ₐ (the association constant) for this complex?

• A) A-B; 10⁵ L/mol
• B) A-B; 10-⁵ L/mol
• C) A-C; 1.5 × 10⁵ L/mol
• D) A-C; 6.7 × 10⁴ L/mol
• E) A-C; 6.7 × 10-⁴ L/mol

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Definitions:

Equilibrium

A state in which opposing forces or influences are balanced, often related to chemical reactions reaching a state where the forward and reverse reaction rates are equal.

Cells

The basic structural, functional, and biological units of all known living organisms, acting as the building blocks of life.

Vitamins

Organic compounds that are necessary in small amounts for the body to maintain health, usually obtained from food, as most cannot be synthesized by the body.

Coenzymes

Non-protein chemical compounds that bind to an enzyme and help it to catalyze a reaction.