You Would Like to Clone a Gene by PCR

question 1

Multiple Choice

You would like to clone a gene by PCR. The sequence of the two ends of the coding strand for the gene is provided below. Which following pair of primers would direct the amplification of this gene? All sequences are written from 5? to 3?.

ATGAAATCTACGTTTCAC……CCCCCAGTACCCCCCTTA

• A) ATGAAATCTACGTTTCAC ; CCCCCAGTACCCCCCTTA
• B) ATGAAATCTACGTTTCAC ; TAAGGGGGGTACTGGGGG
• C) TACTTTAGATGCAAAGTG; ATTCCCCCCATGACCCCC
• D) TACTTTAGATGCAAAGTG ; CCCCCAGTACCCCCCTTA
• E) GTGAAACGTAGATTTCAT; ATTCCCCCCATGACCCCC

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Definitions:

Purchasing Power Parity

An economic theory that compares different countries' currencies through a "basket of goods" approach to assess the relative value of currencies.

Mexican Peso

The currency of Mexico, used as a medium of exchange within the country.