Two Segments (S1 and S2) in a Viral Protein Are

question 26

Multiple Choice

Two segments (S1 and S2) in a viral protein are suspected to be responsible for the nuclear localization of the protein in infected human cells. You have engineered a plasmid to encode the green fluorescent protein (GFP) fused to either or both of these peptide segments, and have introduced the plasmid into the cells. After the expression of the fusion protein is induced, you visualize the cells using a fluorescence microscope equipped with filters appropriate for detection of GFP. Your results are presented in the following schematic drawings in which the GFP signal is represented in green. Which of the following is more consistent with these observations?

• A) Both S1 and S2 are required for nuclear localization.
• B) S1 is required and sufficient for nuclear localization.
• C) S2 is required and sufficient for nuclear localization.
• D) S1 is required but not sufficient for nuclear localization.
• E) S2 is required but not sufficient for nuclear localization.

---

Definitions:

Maturity

The date on which the principal of a debt is due. Also the time from the present until that date.

Annual Interest Rate

The percentage of interest that will be charged or earned on an investment or loan on an annual basis.

Prompt Payment Discount

A discount offered to customers for paying their invoices before a specified due date, used as an incentive for early payment.

Annual Rate

A periodic interest rate calculated over a year, often representing the cost of borrowing money or the earnings from an investment.