Electron Microscopy Samples Are Often Chemically Fixed Before Dehydration, Resin-Embedding

question 6

Multiple Choice

Electron microscopy samples are often chemically fixed before dehydration, resin-embedding, and sectioning. But they can also be "fixed" by rapid freezing, in a way that precludes ice-crystal formation, to ensure minimal damage to the original cell structures. How can this be done?

• A) High-pressure cooling
• B) Plunging into liquid nitrogen
• C) Spraying with a jet of liquid propane
• D) Contact with a copper block cooled by liquid helium
• E) All of the above

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