Atomic Force Microscopy (AFM) Is Used in an Experiment to Unfold

question 3

Short Answer

Atomic force microscopy (AFM) is used in an experiment to unfold a multidomain protein by applying mechanical force. The protein contains several copies of an immunoglobulin domain that are unfolded one by one as the two ends of the molecule (one attached to a cover slip, and the other to the AFM tip) are being pulled apart, resulting in the "sawtooth" force-extension curves shown below. The same experiment is done twice, once in the presence and once in the absence of a chaperone protein that stabilizes the immunoglobulin domains. Answer the following questions based on this graph.

-According to the force-extension graph, how many immunoglobulin domains are unfolded in each of these experiments? Write down the number as your answer, e.g. 9.

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Lend

To provide money or resources to another party with the expectation of future repayment.

Borrow

The act of receiving something with the intent to return it, often referring to money in the context of loans where interest may be charged.

Standard Deviations

A statistic that measures the dispersion of a dataset relative to its mean, used to quantify the amount of variation or spread of a set of data values.