Rough Microsomes Can Be Subjected to a "Salt Extraction" Procedure

question 33

Multiple Choice

Rough microsomes can be subjected to a "salt extraction" procedure in which a high salt concentration is used to remove membrane-associated ribosomes and peripheral proteins. Such salt-extracted microsomes are known to be translocation-incompetent, meaning that when present co-translationally in vitro, they fail to protect translated proteins from protease digestion. However, adding back an 11S particle (S is the sedimentation coefficient) purified from the salt-wash fraction is sufficient to restore the protein-translocation activity of the salt-extracted microsomes. Which of the following do you think is true regarding the 11S particle?

• A) It is composed of 21 proteins.
• B) It is a digestion product of ER-associated ribosomes.
• C) It is normally assembled in the nucleus and exported to the cytoplasm by exportins.
• D) It is an ER integral membrane protein that can interact with the translocon.
• E) It requires high salt concentration for its function in vivo.

Understand the structural differences and functionalities of various muscle types.

Know the basic structure and growth patterns of bones.

Learn about the biochemical processes involved in muscle energy utilization.

Identify and understand the components involved in muscle contraction at the molecular level.

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