The Immortalized Non-Malignant Mouse Cell Line NIH-3T3 Was Derived from Normal

question 21

Multiple Choice

The immortalized non-malignant mouse cell line NIH-3T3 was derived from normal mouse fibroblasts in the early 1960s. These cells are able to readily take up exogenous DNA and are prone to transformation by cancer-causing agents, including some retroviruses. DNA extracted from a human bladder carcinoma line is able to transform these cells, as judged by a significant increase in the number of foci (cell clumps) in the cell-culture plates when the DNA is added. The malignant cells contain human DNA, and the DNA can be shown by sequence analysis to contain a single mutant gene that is present in the original bladder carcinoma cell line. The gene codes for a monomeric G protein and was one of the first cancer-critical genes to be identified in this way. The protein encoded by this gene is …

• A) Src
• B) Ras
• C) Myc
• D) p53
• E) Apc

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Definitions:

Urinary Function

The ability of the kidneys and urinary tract to filter and eliminate waste from the body.

Self-Care Deficit

A condition or situation where an individual is unable to perform essential self-care activities due to physical, mental, or emotional limitations.

Impaired Self-Esteem

A condition where an individual has negative perceptions about their own worth or value, often leading to feelings of inadequacy, inferiority, or unworthiness.

Oliguria

A medical condition defined by the production of abnormally small amounts of urine, often indicating renal issues.