From an Immortalized Human HeLa Cell Line with Wild-Type P53

question 41

Short Answer

From an immortalized human HeLa cell line with wild-type p53 genes, you have derived a line that lacks both copies of the gene. You then treat the original and derived cells with the anticancer drug doxorubicin, which can activate the p53 pathway in the cell by stalling DNA replication forks and inducing double-strand breaks in DNA. You measure cell proliferation in the presence of different doses of the drug in each of the two cell lines, and plot the results as shown in the graph below. Which cell line (A or B) do you expect to be the original HeLa line? Write down A or B as your answer.

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Definitions:

Manufacturing Overhead

Indirect costs associated with manufacturing, including expenses related to operating the factory like utilities, depreciation, and maintenance.

Machine-Hours

A metric indicating the cumulative operational duration of machinery within a specified time frame, often utilized in calculating overhead rates.

Predetermined Overhead Rate

A rate calculated before the period begins by dividing the estimated total overhead costs by an estimated allocation base, used to assign overhead costs to products or job orders.

Manufacturing Cost

The total expense incurred in the process of producing goods, including raw materials, labor, and overhead.