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From an Immortalized Human HeLa Cell Line with Wild-Type P53

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From an immortalized human HeLa cell line with wild-type p53 genes, you have derived a line that lacks both copies of the gene. You then treat the original and derived cells with the anticancer drug doxorubicin, which can activate the p53 pathway in the cell by stalling DNA replication forks and inducing double-strand breaks in DNA. You measure cell proliferation in the presence of different doses of the drug in each of the two cell lines, and plot the results as shown in the graph below. Which cell line (A or B) do you expect to be the original HeLa line? Write down A or B as your answer. From an immortalized human HeLa cell line with wild-type p53 genes, you have derived a line that lacks both copies of the gene. You then treat the original and derived cells with the anticancer drug doxorubicin, which can activate the p53 pathway in the cell by stalling DNA replication forks and inducing double-strand breaks in DNA. You measure cell proliferation in the presence of different doses of the drug in each of the two cell lines, and plot the results as shown in the graph below. Which cell line (A or B) do you expect to be the original HeLa line? Write down A or B as your answer.

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Definitions:

Social Anxiety Disorder

A chronic mental health condition where social interactions cause irrational anxiety and fear.

Generalized Anxiety Disorder

A mental health disorder characterized by persistent and excessive worry about various aspects of life, leading to significant distress or impairment.

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A framework for understanding mental processes such as perception, memory, language, and problem-solving, emphasizing the importance of internal mental states in behavior.

DSM-5

The fifth edition of the Diagnostic and Statistical Manual of Mental Disorders, a widely used handbook for diagnosing mental disorders.

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