The Best Primers for the PCR Reaction Have the Following

question 29

Multiple Choice

The best primers for the PCR reaction have the following feature:

• A) They have a high G − C content.
• B) They have a high A − T content.
• C) They should be palindromic.
• D) The AT\GC ratio does not matter.
• E) They should anneal rapidly, before the larger DNA strands reanneal.

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Definitions:

Equation

A mathematical statement asserting the equality of two expressions, typically involving variables, and solved to find the values of those variables.

Reciprocal

The multiplicative inverse of a number or expression; for any nonzero number x, its reciprocal is 1/x.

Number

A mathematical object used to count, measure, and label.

Extraneous Solutions

Solutions obtained in the process of solving an equation that do not satisfy the original equation.