Why Are Numerous Replication Cycles - Each Performed at Three

question 90

Multiple Choice

Why are numerous replication cycles - each performed at three difference temperatures - a necessary part of a PCR reaction?

• A) The initial cycles do not produce many copies of the target sequence, but since the number of molecules produced doubles with each cycle, millions of copies are produced after 20-30 cycles.
• B) The initial cycles amplify DNA slowly, but as the temperature increases, DNA polymerase works more quickly to produce millions of copies of the target sequence.
• C) The bacteria used in the reaction divide with each cycle, replicating the target DNA.
• D) Only non-target DNA is made during the first 10 cycles, while only cycles 11-20 specifically target the gene of interest.
• E) Once the enzyme starts working, it must continue until there are no remaining nucleotides.

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