A Chemist Was Performing a Spectroscopy Experiment on a Fluorescent

question 29

Multiple Choice

A chemist was performing a spectroscopy experiment on a fluorescent dye to examine the influence of concentration on absorbance. To accomplish this, the chemist created a stock solution of a certain concentration of dye using 10 mL of water (H₂O) . Then, a serial dilution was performed by taking 1 mL of the stock solution and diluting it with 9 mL of water for a total solution volume of 10 mL. 1 mL was taken from Solution 2 and diluted with 9 mL water to create Solution 3. The serial dilution was performed through Solution 5, as shown in the diagram below. If the dye concentration in Solution 5 was 0.5 g/mL, what was the concentration of the dye in Solution 2?

• A)50 g/mL
• B)500 g/mL
• C)5 gmL
• D)5,000 g/mL

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