Microbiologists Are Investigating Sugar Metabolism in Wild-Type and Mutant Strains

question 186

Multiple Choice

Microbiologists are investigating sugar metabolism in wild-type and mutant strains of Escherichia coli.  Both strains are found to grow viable colonies on lactose-containing media.  Each strain is then cultured on a new growth medium containing only glucose.  Representative colonies of each strain from the new media undergo Western blot processing using a fluorescently labeled probe specific for β-galactosidase.  Wild-type bacterial colonies are found to contain only trace quantities of β-galactosidase.  However, the mutant colonies express significant amounts of β-galactosidase.  Further analysis reveals that the variant strain contains a mutation that inhibits the binding of a certain protein to its regulatory sequence.  In which of the following locations did this mutation most likely occur?

• A)Activator protein (CAP) binding site
• B)Operator locus
• C)Promoter region
• D)Activator protein (CAP) gene
• E)RNA polymerase cistron

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