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To Identify the Molecule That Accepts CO₂, Calvin and Benson

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To identify the molecule that accepts CO₂, Calvin and Benson manipulated the carbon-fixation cycle by either cutting off CO₂ or cutting off light from cultures of photosynthetic algae. They then measured the concentrations of various metabolites immediately following the manipulation. How would these experiments help identify the CO₂ acceptor? Study the figure above to help you in determining the correct answer.


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