Use the Following Information to Answer the Next Few Questions

question 17

Multiple Choice

Use the following information to answer the next few questions.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
-Bacteria that do not take up any plasmids would grow on which media?

• A) the nutrient broth only
• B) the nutrient broth and the tetracycline broth
• C) the nutrient broth and the ampicillin broth
• D) the tetracycline broth and the ampicillin broth
• E) all three broths

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Definitions:

Sympathetic

Relating to the sympathetic nervous system, part of the autonomic nervous system that orchestrates the body's rapid involuntary response to dangerous or stressful situations.

Sympathetic

Pertaining to the part of the autonomic nervous system that increases heart rate, constricts blood vessels, and raises blood pressure, typically in response to stress.

Parasympathetic

Part of the autonomic nervous system responsible for conserving energy and bringing the body to a state of rest by slowing the heart rate and increasing intestinal and gland activity.

Autonomic Nervous System

The segment of the nervous system responsible for regulating automatic body functions such as digestion, heartbeat, and breathing rate.