What Information Is Critical to the Success of Polymerase Chain

question 33

Multiple Choice

What information is critical to the success of polymerase chain reaction (PCR) ?

• A)The DNA sequence of the ends of the DNA to be amplified must be known.
• B)The complete DNA sequence of the DNA to be amplified must be known.
• C)The sequence of restriction-enzyme recognition sites in the DNA to be amplified must be known.
• D)The sequence of restriction-enzyme recognition sites in the DNA to be amplified and in the
• Plasmid where the amplified DNA fragment will be cloned must be known.
• E)The plasmid must have a low GC content.

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