The Following Are Steps Involved in Producing Recombinant DNA Using

question 38

Multiple Choice

The following are steps involved in producing recombinant DNA using human DNA and a plasmid. Which one of the following answers best reflects the proper order of these steps? 1. select bacteria that contain human DNA
2) isolate and purify DNA to be recombined
3) add DNA ligase to join human DNA with plasmid
4) cut human DNA and plasmid with restriction enzyme
5) introduce new plasmid to bacteria

• A) 4, 1, 5, 3, 2
• B) 2, 4, 3, 5, 1
• C) 2, 5, 1, 3, 4
• D) 4, 3, 2, 1, 5
• E) 5, 1, 2, 4, 3

---

Definitions:

Indenture

A formal legal agreement, contract, or document, especially one with specific pledges or terms.

Bond Creditors

Creditors who have lent money to a company or government by purchasing bonds issued by the entity.

Written Agreement

A formal document detailing the terms and conditions of a contract agreed upon by two or more parties.

Stripped Bond

A bond that has had its coupon payments removed, leaving only the principal payment, which is received at maturity.