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Consider the separation of Hb 1, Hb 2, and Hb 3 by cation-exchange HPLC.The initial mobile phase pH causes Hb 1 to have the greatest positive charge, Hb 2 to have minimal positive charge, and Hb 3 to be neutral.Predict the order of elution from the stationary phase as the chromatographic run continues from the initial mobile phase to mobile phases with increasing concentrations of cations.(first to elute to last to elute)
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