Which of the Following Approaches to Competitive-Binding Immunoassays Would Be

question 20

Multiple Choice

Which of the following approaches to competitive-binding immunoassays would be most likely to improve the sensitivity of the assay for a specific ligand?

• A)Simultaneously add the labeled and unlabeled ligand to the reaction mixture containing the appropriate antibody; measure bound labeled ligand after a fixed period of time.
• B)Add the unlabeled ligand to the reaction mixture containing the appropriate antibody; allow reaction time; add the labeled ligand and allow a second reaction time; measure the bound labeled ligand
• C)Add the labeled ligand to the reaction mixture containing the appropriate antibody; allow reaction time; add the unlabeled ligand and allow a second reaction time; measure the bound labeled ligand.
• D)None of the above; the order of addition of the labeled ligand, the unlabeled ligand, and the antibody will have no influence on the sensitivity of the assay.

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Definitions:

Fixed

Expenses that remain constant for a period of time and do not vary with production levels or sales volume.

Specified Levels

Predetermined benchmarks or criteria that need to be met within certain contexts, such as quality standards or performance thresholds.

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