As a Preliminary Step Before Performing the Assay, Some Competitive-Binding

question 1

Multiple Choice

As a preliminary step before performing the assay, some competitive-binding assays either adjust the pH of the patient sample or add an excess of a ligand that is similar to that being measured.Which of the following correctly describes the purpose for these preliminary steps?

• A)to release ligands that are bound by endogenous proteins prior to analysis to allow measurement of the total ligand concentration
• B)to stabilize ligands that are bound by endogenous proteins prior to analysis in order to prevent these ligands from interfering with free-ligand concentration measurement
• C)to release ligands that are bound by endogenous proteins prior to analysis in order to remove these ligands and prevent interference with the concentration measured
• D)to stabilize ligands that are bound by endogenous proteins prior to analysis in order to specifically measure the concentration of bound ligand only

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