The Assumption Underlying Real-Time PCR Is That the Amount of Fluorescence

question 17

Multiple Choice

The assumption underlying real-time PCR is that the amount of fluorescence in the reaction chamber is:

• A)directly proportional to the amount of amplicon present in the PCR vessel
• B)inversely proportional to the amount of amplicon present in the PCR vessel
• C)directly proportional to the amount of target template present at the beginning of the reaction
• D)both a and c

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Definitions:

Monte Carlo Simulation

A computational algorithm that uses repeated random sampling to obtain numerical results, often used in risk analysis and decision making.

Random Numbers

Numerical values generated in such a way that each possible number has an equal chance of being selected, often used in simulations and cryptographic applications.

Time Compression

Techniques or strategies aimed at reducing the time needed to complete processes or activities, thereby increasing efficiency.

Trial and Error

A problem-solving method involving repeated, varied attempts which are continued until success, or until the practicer stops trying.