DNA Sequencing by Synthesis Is a Powerful Contemporary Technique That

question 33

Multiple Choice

DNA sequencing by synthesis is a powerful contemporary technique that allows the generation of up to 600 gigabases (Gb) of sequence per run. Which process is part of sequencing by synthesis?

• A) The sequencing products are separated by polyacrylamide gel electrophoresis.
• B) [32P]-DNA fragments are developed using X-ray film or phosphoimaging.
• C) The release of pyrophosphate during the incorporation of dNTPs into a growing DNA chain is detected by a complex reaction system that emits light from oxyluciferin.
• D) About one thousand identical copies of each individual template DNA form a tight cluster (at the mm-level) on the surface of a flow cell prior to sequencing.
• E) Whole large DNA molecules, such as plasmids or BACs, are used as templates.

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