What Information Is Critical to the Success of PCR Itself

question 11

Multiple Choice

What information is critical to the success of PCR itself?

• A)The DNA sequence of the ends of the DNA to be amplified must be known.
• B)The complete DNA sequence of the DNA to be amplified must be known.
• C)The sequence of restriction enzyme recognition sites in the DNA to be amplified must be known.
• D)The sequence of restriction enzyme recognition sites in the DNA to be amplified and in the plasmid where the amplified DNA fragment will be cloned must be known.

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Definitions:

Normality

A statistical condition where data is symmetrically distributed around the mean, following a bell-shaped curve known as the normal distribution.

Null Hypothesis

The default hypothesis that there is no significant difference or relationship between specified populations, any observed difference being due to sampling or experimental error.

Directional Alternative

A hypothesis that outlines the anticipated direction in the variation or association among variables.

T-statistic

A T-statistic is used in statistical testing to determine the significance of the difference between the means of two groups relative to the spread or variability of their scores.