Assume That You Are Trying to Insert a Gene into a Plasmid

question 36

Multiple Choice

Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

• A)insert the fragments cut with X directly into the plasmid without cutting the plasmid.
• B)cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid.
• C)cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.
• D)cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.
• E)cut the plasmid with enzyme X and then insert the gene into the plasmid.

Understand the importance and process of journal entries in accounting.

Grasp the concept and purpose of a general ledger and subsidiary ledgers.

Comprehend the essential principles of designing and developing an accounting information system, including cost effectiveness and flexibility.

Understand the role of data collection, processing, and dissemination in an accounting information system.

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Definitions:

Middle School

An educational institution serving students typically between the ages of 11 and 14, bridging the transition from elementary school to high school.

Zuckerman

Refers to Marvin Zuckerman, an American psychologist known for his research on sensation seeking as a personality trait.

Sensation Seeking

A trait describing the tendency to seek out novel, varied, complex, and intense sensations and experiences, and the willingness to take risks for the sake of such experiences.

Adventure

An exciting or remarkable experience involving uncertain outcomes and risks.