You Are Trying to Produce a CDNA Library Starting from MRNA

question 13

Multiple Choice

You are trying to produce a cDNA library starting from mRNA in skeletal muscle cells. You add the following components to a tube and let the reaction proceed: skeletal muscle mRNA, free nucleotides, and DNA polymerase. After inspecting the products of the reaction, you do not find any cDNA molecules at all. What is the most likely explanation for this result?

• A)You used mRNA instead of genomic DNA as the source material.
• B)You used DNA polymerase instead of reverse transcriptase.
• C)cDNA libraries can only be made from skin cell mRNA molecules.
• D)You forgot to add a radiolabeled nucleic acid probe to the tube.

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Definitions:

Repressors

Proteins that bind to DNA and inhibit the transcription of certain genes.

Activators

Molecules that increase the activity of enzymes or other proteins, often playing critical roles in various biological pathways.

Homeotic Genes

Genes that control the development of anatomical structures in various organisms by determining the identity of body parts.

Transcription Factors

Proteins that bind to specific DNA sequences to control the rate of transcription of genetic information from DNA to messenger RNA.