A Researcher Wants to Knock Down Gene Expression in Mouse

question 10

Multiple Choice

A researcher wants to knock down gene expression in mouse embryos at 12 days of development.Which approach could be used to accomplish this?

• A) Injection of morpholinos into the two-cell embryo
• B) Injection of RNAi into the two-cell embryo
• C) Breeding of two transgenic mice, one with the gene of interest flanked by LoxP sites and another with an inducible promoter driving cre recombinase, and treat pregnant females at the desired time with an appropriate reagent such as Tamoxifen
• D) Breeding of two transgenic mice, one with the gene of interest flanked by LoxP sites and another with the appropriate tissue-specific promoter driving cre recombinase
• E) Generating a transgenic mouse using mutated DNA homologous to the desired target site in the gene of interest

Describe the sexual response cycle as researched by Masters and Johnson.

Explain the significance of the Thematic Apperception Test in assessing motives related to achievement and affiliation.

Recognize the role of prenatal factors in determining sexual orientation.

Understand the continuum of sexual orientation and its biological basis.

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Definitions:

INTERHEART Study

A large international study that analyzed the risk factors associated with heart attack in various populations around the world.

CVD Risks

Factors that increase the likelihood of developing cardiovascular diseases, including high blood pressure, smoking, and diabetes.

Low Education

Refers to a lower level of formal schooling or academic achievement, often associated with various social and health disadvantages.

HDL Levels

High-density lipoprotein (HDL) levels indicate the amount of 'good' cholesterol in the blood, which helps reduce heart disease risk.