A Researcher Wants to Knock Down Gene Expression in Mouse

question 10

Multiple Choice

A researcher wants to knock down gene expression in mouse embryos at 12 days of development.Which approach could be used to accomplish this?

• A) Injection of morpholinos into the two-cell embryo
• B) Injection of RNAi into the two-cell embryo
• C) Breeding of two transgenic mice, one with the gene of interest flanked by LoxP sites and another with an inducible promoter driving cre recombinase, and treat pregnant females at the desired time with an appropriate reagent such as Tamoxifen
• D) Breeding of two transgenic mice, one with the gene of interest flanked by LoxP sites and another with the appropriate tissue-specific promoter driving cre recombinase
• E) Generating a transgenic mouse using mutated DNA homologous to the desired target site in the gene of interest

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Definitions:

Effective Interest Method

An accounting technique used to allocate the interest expense or income of a bond over its life in a way that yields a constant rate of return.

Interest Expense

The cost incurred by an entity for borrowed funds, which is considered a non-operating expense on the income statement.

Semiannually

Occurring twice a year, typically used in the context of payments, interest accruals, or reporting periods.

Effective-interest Method

A method of amortizing bond discounts or premiums that reflects the constant rate of interest over the life of the bond with respect to its carrying value at the beginning of each interest period.