A Researcher Wants to Knock Down Gene Expression in Mouse

question 10

Multiple Choice

A researcher wants to knock down gene expression in mouse embryos at 12 days of development.Which approach could be used to accomplish this?

• A) Injection of morpholinos into the two-cell embryo
• B) Injection of RNAi into the two-cell embryo
• C) Breeding of two transgenic mice, one with the gene of interest flanked by LoxP sites and another with an inducible promoter driving cre recombinase, and treat pregnant females at the desired time with an appropriate reagent such as Tamoxifen
• D) Breeding of two transgenic mice, one with the gene of interest flanked by LoxP sites and another with the appropriate tissue-specific promoter driving cre recombinase
• E) Generating a transgenic mouse using mutated DNA homologous to the desired target site in the gene of interest

Understand the psychosocial and familial factors that may contribute to the development of feeding and eating disorders.

Comprehend the diagnostic criteria and subtypes of anorexia nervosa, bulimia nervosa, binge-eating disorder, and obesity, along with their prevalence rates and risk factors.

Identify and understand the biological factors implicated in feeding and eating disorders.

Be informed on the efficacy of drug treatments for bulimia nervosa and other eating disorders.

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Definitions:

Similarity

The degree to which two or more things resemble each other in terms of attributes, characteristics, or qualities.

Fundamental Attribution Error

The tendency to overemphasize personality traits and underestimate situational factors when explaining others' behaviors.

Availability Heuristic

A cognitive rule of thumb that says that the perceived probability of an event or the importance assigned to it is based on its availability in memory.

Augmenting Principle

A principle in attribution theory suggesting that if an event occurs despite the presence of strong opposing forces, we should attribute the event to the actions of the agent.