You Have Isolated DNA for PCR and Accidentally Added Topoisomerase

question 42

Multiple Choice

You have isolated DNA for PCR and accidentally added topoisomerase instead of polymerase to your sample. What would you expect if you ran this DNA sample on a gel?

• A)The DNA would show increased supercoiling and have a lower electrophoretic mobility.
• B)The DNA would show decreased supercoiling and have a higher electrophoretic mobility.
• C)The DNA would show increased supercoiling and have a higher electrophoretic mobility.
• D)The DNA would show decreased supercoiling and have a lower electrophoretic mobility.
• E)The DNA would show decreased supercoiling, but electrophoretic mobility will be unaffected.

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Definitions:

Activity Analysis

The process of breaking down and examining activities within a process or system to improve efficiency and productivity.

Unit Fixed Costs

The consistent expenses incurred by a company for each unit produced that do not change with the volume of production.

Level of Activity

A measure of the volume of production or operations, often influencing costs and profitability in a business.

Variable Costs

Expenses that vary in relation to the amount of products or services a company generates.