You Are Trying to Prepare a DNA Karyotype,but This Time

question 5

Multiple Choice

You are trying to prepare a DNA karyotype,but this time you forgot to add trypsin to your chromosome preparation.What effect would you expect to see?

• A)Dyes would stain the chromosomes darker because more proteins are present to take up the dye.
• B)Dyes would not stain the chromosomes as expected,because proteins would not be removed from the chromatin and so would block the dye from accessing the DNA.
• C)Chromosomes do not condense properly,so it would be very difficult to isolate individual chromosomes.
• D)Chromosomes would be degraded quickly because the trypsin is necessary to promote DNA stability by adding more scaffold proteins to the chromatin.
• E)The chromosomes would be more difficult to pair up because the trypsin acts as a dye,creating a banding pattern that distinguishes between chromosomes.

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Definitions:

Direct Labor-hours

The total hours worked by employees directly involved in the production process, used as a basis for allocating labor costs to products or job orders.

Variable Overhead Efficiency Variance

The difference between the expected (standard) and actual variable overhead costs based on the actual level of an activity.

Variable Manufacturing Overhead

The portion of manufacturing overhead costs that varies directly with the volume of production, such as utilities for machinery.

Variable Overhead Efficiency Variance

The difference between the actual variable overhead incurred and the standard cost of variable overhead allocated for the actual production achieved.