In Reverse Genetics, What Is the Correct Order in Which

question 36

Multiple Choice

In reverse genetics, what is the correct order in which the experimenter proceeds?

• A)silencing the genes in question using RNAi, followed by generating gain-of-function alleles
• B)screening genes via PCR, followed by deletion of the flanking sequences
• C)selection of a phenotypic alternative for the gene in question and sequencing its DNA
• D)induction of and selection for a mutant allele with a known sequence, followed by screening for mutations of interest
• E)random bombardment of the DNA with a known mutagen, followed by observation of offspring for newly acquired traits

---

Definitions:

Flashbacks

Sudden, intense, involuntary re-experience of past memories or traumatic events, often a symptom of PTSD.

PTSD

Post-Traumatic Stress Disorder, a mental health condition triggered by experiencing or witnessing a terrifying event, characterized by flashbacks, nightmares, and severe anxiety.

Reexperiencing

The involuntary reliving of traumatic events, often a symptom of PTSD.

Wounds Dehisced

A surgical complication where a wound ruptures along a surgical incision, often due to improper healing or infection, leading to the separation of the previously joined edges.