In Looking for a Gene Responsible for Albinism in Humans,a

question 31

Multiple Choice

In looking for a gene responsible for albinism in humans,a group of researchers decided that at least one form of this condition was due to a lack of the enzyme tyrosinase.With this decision as a starting point,which of the following series of steps was most feasible?

• A)Sequence the amino acids of the protein,use rabbits to make an antibody to the protein,purify and fluorescently label the protein,and hybridize the antibody to a cDNA library.
• B)Sequence the protein,use the code table to figure out the best RNA sequence for it,synthesize the RNA,and use the RNA as a probe in cells from albino individuals.
• C)Radioactively tag the enzyme and use it as a probe to identify the biochemical reaction in which it functions,work out the metabolic pathway involved,and find cells in albinos that cannot carry out these reactions.
• D)Sequence the protein,work out the cDNA that codes for it,label the cDNA code,and use a FISH study to localize the gene on a karyotype.
• E)Sequence the appropriate region from albino and non-albino cells and compare the sequences or do subtractive hybridization to see where there is a deletion.

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Definitions:

Organic Reaction

A process that involves a structural rearrangement of organic molecules, leading to the formation of new organic compounds.

Stereochemistry

The study of the spatial arrangement of atoms in molecules and the effects of these arrangements on the chemical and physical properties of substances.

Regiochemistry

The branch of chemistry that deals with the direction or orientation of chemical bond formation or breaking during a chemical reaction.

Kinetic Conditions

Conditions that affect the rate of a chemical reaction, focusing on temperature and concentration of reactants.