The PCR (Polymerase Chain Reaction)protocol That Is Currently Used in Laboratories

question 10

Multiple Choice

The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park,in Wyoming.This organism contains a heat-stable form of DNA polymerase known as Taq polymerase,which continues to function even after it has been heated to 95ᵒC.Why would such a heat-stable polymerase be beneficial in PCR?

• A) Each cycle includes a "hot" saturation phase (95°C), which allows the primers to anneal to the target DNA.
• B) Each cycle includes a "hot" denaturation phase (95°C), which serves to sterilize the culture.
• C) Each cycle includes a "hot" denaturation phase (95°C), which activates the Taq polymerase.
• D) Each cycle includes a "hot" denaturation phase (95°C), which separates the hydrogen bonds that hold the strands of the template DNA together.
• E) More than one of the above are correct.

---

Definitions:

Globalization

The process by which businesses or other organizations develop international influence or start operating on an international scale, resulting in increased interconnectedness of the world's economies, cultures, and populations.

Theological Stage

The earliest stage in Comte's law of three stages, characterized by human understanding of the world as explained by supernatural beings and religious leaders.

Metaphysical Stage

A phase in Auguste Comte’s Law of Three Stages where human thought is dominated by abstract and speculative reasoning, seeking the essence of phenomena rather than their physical or empirical properties.

Positive Stage

A term in sociology introduced by Auguste Comte referring to the final stage of societal evolution where explanations of phenomena are based on scientific observations, experiments, and logic.