You Perform a Cell Free Translation Experiment Like Nirenberg and Matthaei,but

question 1

Multiple Choice

You perform a cell free translation experiment like Nirenberg and Matthaei,but you forget to write down what nucleotides you added to make the mRNA.You precipitate the translated polypeptides and measure the relative amount of radiolabeled amino acids incorporated into them. You get 25% proline,25% threonine,12.5% glutamine,12.5% lysine,12.5% asparagine,and 12.5% histidine. What nucleotides and in what % did you add to make the mRNA?

• A) Equal amounts of A, C, U, and G
• B) 50% C and 50% A
• C) 70% C and 30% A
• D) 50% C and 50% U
• E) 50% A and 50% G

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Definitions:

Discoloration

A change in color of a body part, typically indicative of injury or disease.

Pulsation

A rhythmic throbbing or beating, often related to the heartbeat or the flow of blood within the arteries.

Foreign Bodies

Objects or substances found within the body that are not naturally produced by it, which can sometimes lead to infection or injury.

Sterile Gauze

A medical dressing made from a thin, transparent fabric that has been sterilized to prevent infection when applied to wounds.