Why Must Double-Stranded DNA Probes Be Denatured When Used in Northern

question 68

Multiple Choice

Why must double-stranded DNA probes be denatured when used in northern blot experiments?

• A) to ensure that they are properly radioactively labeled or tagged with a dye
• B) to ensure that the DNA coding strand hybridizes to RNA on the membrane
• C) to ensure that both DNA strands bind the single-stranded RNA on the membrane
• D) all of the above
• E) none of the above

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Definitions:

Animal Trainers

Professionals who use behavior modification techniques to teach animals specific responses to specific conditions or stimuli.

Successive Approximations

A technique in behavior modification that rewards behaviors that progressively get closer to the desired one.

Operant Conditioning

A learning process in which the consequences of an action determine the likelihood of that action being repeated.

Negative Reinforcer

A stimulus whose removal or avoidance after a behavior increases the likelihood of that behavior being repeated.